In a recent paper published in Journal of Insect Physiology we describe the physiological and transcriptional changes occurring during RCH in Drosophila. In a rare attempt to cover several orders of biological organization we study RCH at the level of transcription, protein translation and try to associate these changes to activity of up-regulated proteins and their resulting metabolites.
The main finding of the study was that increased abundance of a single protein glycogen phosphorylase was associated with increased levels of glucose, but interestingly, these changes were not associated with increased transcription of GP transcripts or of increased GP activity when measured at the level of the protein.
The rapid cold hardening response of Drosophila melanogaster: Complex regulation across different levels of biological organization
Johannes Overgaard, Jesper Givskov Sorensen, Emmanuelle Com, Herve Colinet